Introduction to the use of biological buffer MOPSO buffer (CAS number 68399-77-9)

MOPSO is an organic acid buffer with the molecular formula C7H15NO4S, a molecular weight of 223.26, and a pH range of 7.2-7.6. It can be used in biochemical experiments to buffer biochemical enzyme reactions and protein purification processes. This article will introduce the advantages of MOPSO and its application in biochemical experiments.

Advantages of MOPSO

MOPSO has the following advantages compared to commonly used buffering agents HEPES and Tris HCl in living organisms:

1. MOPSO has a more stable buffering capacity, making it less prone to pH fluctuations during the reaction process;

2.MOPSO has low ionic strength and high hydrophobicity, and will not affect the functions of many enzymes and proteins;

3.MOPSO has a good protective effect on many important biomolecules, such as DNA, RNA, and enzymes;

4. MOPSO will not interact with some commonly used gel blockers, dyes and metal ions.

Application of MOPSO in Biochemical Experiments

1. Buffer solution for enzyme reaction

MOPSO can be used as an ideal buffer for various biochemical enzyme reactions, such as protein kinases, nucleotide phosphatases, esterases, isomerases, etc. In addition, MOPSO can also be used to catalyze the acetyl transfer reaction of deacetylated coenzyme A.

2. Protein purification

MOPSO can be used to purify proteins, effectively protecting their structure and function, and reducing the risks caused by changes in acidity or alkalinity.

3. RNA electrophoresis buffer

MOPSO can be used as an RNA electrophoresis buffer. Because MOPSO can inhibit the electrophoretic migration of a small amount of DNA, with minimal impact on RNA electrophoresis, it can maintain RNA integrity and better isolate RNA.

Desheng is a manufacturer of MOPSO buffering agents. Currently, the MOPSO produced is jointly produced by the company's internal R&D team, with excellent product quality and high purity, which can meet many experimental needs. We can provide technical guidance and professional after-sales service. If you have any purchasing needs, please feel free to contact us at any time.