Biological buffer piperazine-1,4-diethylsulfonic acid monosodium salt PIPES-NA basic information

Piperazine 1,4-diethylsulfonic acid monosodium salt, with the chemical formula C8H17N2NaO6S2, is an organic compound that appears as a white powder and is easily soluble in water. PIPES sodium salt is a stable buffering agent with a high pKa value, which can provide a stable pH environment and help maintain the activity of biomolecules. It plays a key role in fields such as molecular biology, cell culture, and electrophoresis technology.

The use of PIPES sodium salt

As a biological buffering agent, PIPES sodium salt plays an important role in multiple fields. Firstly, in molecular biology research, it is used to maintain the structural and functional stability of proteins. Secondly, in cell culture, it can provide a suitable pH environment for cells, which helps with cell growth and division. In addition, PIPES sodium salt is widely used in electrophoresis technology as a support medium for separating biomolecules such as proteins and nucleic acids.

Advantages of PIPES Sodium Salt

1. High stability: PIPES sodium salt has a high pKa value and can provide a stable pH environment over a wide pH range. This makes it perform well in various biological experiments and less susceptible to external environmental influences.

2. Good cell compatibility: PIPES sodium salt acts as a buffer and has no toxic effect on cells, making it safe to use in cell culture. It can maintain a suitable pH value, promote cell growth and division.

3. Good separation effect: PIPES sodium salt has good separation effect in electrophoresis technology. It can provide a good separation medium to effectively separate biomolecules such as proteins and nucleic acids.

Precautions for using PIPES sodium salt

1. Storage and stability: PIPES sodium salt should be stored in a dry, cool place, away from direct sunlight and high temperature environments. Under appropriate conditions, its stability is good, but its quality still needs to be checked regularly.

2. Dosage and preparation: When preparing PIPES sodium salt, the appropriate concentration should be accurately weighed and prepared according to experimental requirements. Excessive or insufficient amounts may lead to inaccurate experimental results.

3. Avoid cross contamination: During the experimental process, the sterility and purity of the reagents should be ensured. Avoid direct contact with buffer powder or liquid reagents by hand to reduce the risk of cross contamination.

4. Cytotoxicity testing: For applications involving cell culture, cytotoxicity testing should be conducted before using PIPES sodium salt. Ensure that the buffer is non-toxic or low toxic to cells to ensure the reliability of experimental results.

5. Personal protection: When using PIPES sodium salt for related experimental operations, attention should be paid to taking protective measures, such as wearing masks, goggles, protective clothing, gloves, etc., to prevent PIPES sodium salt solution or powder from getting on the skin or inhaling into the body, thereby endangering physical health.

6. Waste disposal: The PIPES sodium salt waste liquid after use should be disposed of according to laboratory waste disposal regulations to ensure that it does not cause environmental pollution.

Hubei Xindesheng Material Technology Co., Ltd. is a high-quality manufacturer specializing in the production of in vitro diagnostic reagents. Since its establishment in 2005, the company has developed a wide range of products, from a single blood collection tube additive to a variety of diversified reagents such as biological buffering agents, chemiluminescence reagents, and chromogenic substrates. Desheng has a professional R&D team and quality inspection team to strictly control the quality of every batch of products sold, while reducing costs and allowing every customer to buy cost-effective products. We also welcome elites in the same industry to exchange and learn from us!