You can't help but know about the applications of CHAPS buffer

In the fields of biochemistry and molecular biology, buffering agents play an important role in maintaining the stability of experimental systems and ensuring the integrity of biomolecules. CHAPS, as a type of biological buffer, has a CAS number of 75621-03-3 and a molecular formula of C ∝? H ?? N ? O ? S. From its chemical structure, it is a substance that combines the useful properties of sulfobetaine type and bile salt detergent. At room temperature, CHAPS appears as a white powder solid. Its good water solubility (50mg/mL) makes it easy to operate and prepare solutions in experimental applications, which also gives it a favorable position in many application fields.

Application of CHAPS in membrane protein related research

1. Dissolved membrane proteins: The study of membrane proteins has always been one of the key and difficult points in the field of biochemistry. CHAPS, as a non denaturing stain remover, has excellent performance in this regard. The lipid bilayer of the cell membrane provides a hydrophobic environment for membrane proteins, making the extraction and study of membrane proteins difficult. The zwitterionic structure of CHAPS can interact with membrane proteins, and its hydrophobic portion can bind to the hydrophobic region of the membrane protein, while its hydrophilic portion interacts with the aqueous phase, thereby dissolving the membrane protein from the cell membrane. This gentle dissolution method helps maintain the structural integrity of membrane proteins and provides high-quality samples for subsequent analysis and research.  

2. Destruction of nuclear membrane and proteins: In some studies, it has been found that CHAPS has advantages over common cleaning agents such as NP-40 and Triton X-100. It can gently destroy the nuclear membrane and has a role in studying biomolecules such as proteins and nucleic acids in the nucleus. Meanwhile, CHAPS also performs well in disrupting protein-protein interactions (PPIs). In cells, many proteins form complex network structures through interactions, and studying these interactions is important for understanding the physiological functions and pathological mechanisms of cells.

Protective effect on protein status

During the experimental process, especially when involving protein extraction, purification, and other operations, protein denaturation is easily caused by changes in external conditions. CHAPS can protect the state or conformation of proteins, which makes it irreplaceable in biological research. Whether during cell lysis or subsequent protein purification steps, CHAPS can provide a relatively stable environment for proteins, reducing the risk of protein denaturation caused by physical and chemical factors, thereby maintaining protein activity and function.

Widely applied in the field of biological science research

In the process of cell lysis, a reagent that can both damage the cell membrane and protect the biomolecules inside the cell is needed. CHAPS perfectly meets this requirement. It can quickly and gently lyse cells, releasing various components inside the cells. Meanwhile, due to its protective effect on protein conformation, the released protein can maintain its activity, providing high-quality samples for subsequent analysis and research. This advantage in cell lysis makes CHAPS one of the ideal reagents for many laboratories in cell biology research.  

CHAPS buffer plays an important role in biological science research due to its chemical properties and excellent performance in multiple aspects. Hubei Xindesheng Material Technology Co., Ltd., as a manufacturer of CHAPS buffering agents, specializes in white powder with high purity, wide experimental range, large number of users, and affordable price. If you have purchasing intentions, please feel free to click on the website for consultation at any time!