What is the function of the chromogenic substrate MADB 209518-16-1?

The Chinese name of MADB is N, N-di (4-sulfobutyl) -3,5-dimethylaniline sodium salt, with a CAS of 209518-16-1. It is a highly water-soluble and sensitive peroxidase colorimetric substrate commonly used in laboratories. It can react with peroxidase to form a complex, which can be detected for peroxidase activity through color reaction, thereby evaluating the immune function of cells, the proliferation ability of tumor cells, and the toxicity of environmental pollutants to organisms. Below, we will analyze the role of MADB in detail

1、 Substrate action

The main function of MADB as a chromogenic substrate is to provide a reactive chemical structure for converting the reactive oxygen species generated by peroxidase catalysis into visible products. Because in this process, the structure of MADB has specific binding sites with the reaction products catalyzed by peroxidases, making the binding between the substrate and the enzyme more tight and stable.

2、 Color change effect

When MADB reacts with peroxidase, the substrate is oxidized to an unstable intermediate, which is then converted to a stable product through the reduction of MADB. During this process, the color of the substrate will change, which can be used to evaluate the activity and stability of peroxidase, as more active enzymes produce more intermediates and products, resulting in a more pronounced change in substrate color.

3、 Signal amplification function

In addition to the substrate itself, MADB can also enhance enzyme reaction efficiency through signal amplification. This is because the binding site between MADB and the enzyme is highly specific, and only substrates that conform to specific structures can react with it. Therefore, MADB has a high affinity and can be effectively catalyzed, which is exactly what can be achieved.

In summary, MADB, as a peroxidase colorimetric substrate, has multiple functions that together constitute its unshakable position in colorimetric experiments. At the same time, it also provides researchers with a fast, sensitive, and reliable method to help them complete complex experimental operations.

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