Detailed Introduction to Acridoyl Hydrazide NSP-SA-ADH

Acrylamide hydrazide NSP-SA-ADH, as a reagent that can be used as a chemiluminescence marker, has been proven to have much higher stability, labeling specificity, and detection sensitivity than other luminescent reagents. Due to its mild reaction conditions, rapid luminescence efficiency, and high luminescence intensity, it is widely used in environmental monitoring, biological and pharmaceutical analysis, and has also played a unique role in the diagnosis of many large diseases.

1、 Introduction to Acrylamide Hydrazide NSP-SA-ADH

Acridine ester acridine hydrazide (NSP-SA-ADH), with a yellow powder appearance and a purity of over 98%, has a molecular weight of 740.85. It is commonly packaged in brown bottles and stored at low temperatures in the dark. It is suitable for labeling antigen antibody proteins. Although similar to other products in the acridine series and having an acridine ring, the detection substances are different, and the role played by acridine hydrazide should not be underestimated.

2、 Luminescence mechanism of acridine hydrazide NSP-SA-ADH

The luminescence process of acridine hydrazide is the same as other acridine products, both using an alkaline solution of hydrogen peroxide. Once the molecule is attacked, it will generate an unstable dioxane, which will then decompose into carbon dioxide and N-methylacridone. When it returns to the ground state, it will emit photons containing wavelengths. The entire luminescence process does not require any other auxiliary reagents, effectively reducing background luminescence and improving overall detection efficiency, with minimal external interference factors.

3、 Advantages of Acrylaminoyl Hydrazide NSP-SA-ADH

1. The labeling rate is high because it contains free amino groups, making it suitable for directly coupling polysaccharides, nucleic acids, or proteins containing aldehyde groups.

2. Acrylamide hydrazide has hydrolysis resistance and stability, and its hydrolysis resistance is generally stronger than other acridine compounds. In addition, even in acidic solutions, its performance is very stable. When stored at room temperature for 4 weeks with protein conjugates, its light quantum yield does not decrease, and even at -20 ℃, it can be stored for more than a year.

3. It has hydrophilicity, as the produced acridine hydrazide is in powder form and is prepared into a solution when used. It is required to dissolve in water, so that it can be water-soluble with markers formed by antibodies, nucleic acids, etc., facilitating later luminescence detection.

As a professional manufacturer of chemiluminescent reagents, Desheng sells six series of luminescent reagent products that can meet the different needs of customers. At the same time, acridine ester and acridine hydrazide have brought great convenience to customers due to their high luminescence efficiency and simple operation, so they are highly popular in the market and have become hot selling products. If you have any relevant needs, please click on the website to inquire about details!