The pH range of N - (2-acetamido) -2-iminodiacetic acid (ADA) buffer

N - (2-acetylamino) -2-iminodiacetic acid (ADA) is a multifunctional buffering agent that not only has superior buffering performance, allowing the solution to maintain a stable pH environment, but also has unparalleled advantages over other buffering agents, playing a role in rare fields. Let's take a look together below.

PH range of ADA buffer

The uniqueness of ADA buffer lies in its excellent buffering performance within the pH range of 6.0-7.2. Within this range, he is able to effectively maintain the acid-base balance of the solution, making the experimental conditions more stable.

Multi domain applications

1. Protein crystallization and microtubule polymerization: ADA is widely used in protein crystallization and microtubule polymerization experiments. The appropriate pH range enables ADA buffer to provide a stable acid-base environment in these experiments, helping to maintain the stability of proteins or microtubules.

2. Capillary electrophoresis: As a buffer for capillary electrophoresis, it can provide good separation performance. Especially in the separation of amiodarone and diethylamiodarone, ADA exhibits excellent performance, which helps to improve the accuracy of the experiment.

3. Differential scanning calorimetry: ADA was used as the sample buffer in the differential scanning calorimetry experiment. Its unique properties allow researchers to delve into the properties of samples such as fibroblast growth factor in situations where phosphate or sulfate buffer solutions cannot be studied.

Precautions and advantages

1. PH measurement accuracy: When using ADA buffer, ensure that an accurate pH meter is used for measurement to ensure the reliability of experimental results.

2. Multifunctionality: The versatility of ADA buffer makes it an important role in many biochemical experiments, able to adapt to different experimental requirements.

3. Combining strong metal ions: In terms of chemical properties, ADA is relatively active and has a strong ability to bind metal ions. It can complex with copper, nickel, zinc, calcium, magnesium, manganese, cobalt, etc. It should be noted that ADA buffering agents are generally not recommended in reaction solutions that require metal cations. Other suitable buffering agents should be selected based on experiments.

The N - (2-acetamido) -2-iminodiacetic acid (ADA) buffer has attracted widespread attention for its unique pH range and versatile applications. Its role in protein crystallization, microtubule polymerization, capillary electrophoresis, differential scanning calorimetry and other experiments provides researchers with important experimental tools and superior buffering performance.

As a manufacturer of ADA buffering agents, Desheng can provide raw materials with a purity of over 99%. It is easy to prepare, convenient to store, not prone to deterioration, and reduces performance. At present, we have cooperation with 80% of enterprises or laboratories in the market, and export to up to 100 countries. If you have any relevant intentions, feel free to contact us and give it a try!