Preparation and Use of New Trinder's Reagent DAOS 83777-30-4 Solution

Nowadays, with the improvement of living standards, while enjoying oneself, it inevitably brings some physical problems, such as various high-fat, high blood sugar, high blood pressure, and cardiovascular and cerebrovascular diseases. How can we effectively prevent such diseases? In addition to paying attention to daily dietary habits, routine testing is also necessary. When it comes to testing, it cannot be separated from its raw materials. Next, we will focus on introducing DAOS, a member of the new Trinder's reagent. Why introduce it? Mainly because it is frequently used in our testing projects, but many people are not familiar with it.

DAOS is a color developing raw material used in biological reagent kits, with a CAS number of 83777-30-4. It is a white solid powder with high water solubility. As an aniline analogue, it has good stability and is suitable for biochemical testing experiments. There are several specific project detections:

1. DAOS is used for detecting blood glucose levels

In daily blood testing, glucose measurement is one of the important clinical indicators. The new Trinder's reagent DAOS coupled with 4-aminoantipyrine can be used as a reducing chromogen to determine glucose content.

2. DAOS is used for cholesterol detection

By coupling DAOS and 4-AAP, a color reaction is generated with H2O2 under peroxidase catalysis, and the concentration of total cholesterol can be determined by measuring the absorbance.

3. DAOS is used for triglyceride detection

Triglycerides are hydrolyzed into glycerol and fatty acids in the presence of lipoprotein esterase. Glycerol and ATP are catalyzed by glycerol kinase to produce glycerol-3-phosphate and ADP. Glycerol-3-phosphate and oxygen generate DHAP and H2O2 under the catalysis of glycerol-3-phosphate oxidase (GPO), and the same principle applies to the chromogenic substrate DAOS. The coupling of 4-AAP with H2O2 produces a chromogenic reaction, and the TG concentration is measured.

4. DAOS detection of high-density lipoprotein

When DAOS is used to detect high-density lipoprotein, it reacts through a dual reagent form. One reagent contains polyanions and surfactants, selectively binding to both very low-density lipoprotein and low-density lipoprotein. At the same time, it will inhibit the chemical oxygen demand in reagent 2, and CEH has an effect on VLDH-CH and LDH-CH. Thus, through the action of CEH-COD-POD, combined with measuring absorbance to determine HDL concentration, LDL concentration can be obtained through calculation.

Desheng Biochemical has been specializing in the research and production of Trinder's new reagents for 17 years. As the original manufacturer, it has rich experience. In addition to the color reagent DAO, there are also TOOS, TOPS, ADOS, ADPS, ALPS, DAOS, etc. If you have a need for color reagents, you can click on the official website or call for consultation. Welcome to purchase!