Luminescence analysis of high-purity acridine ester NSP-DMAE-NHS 194357-64-7

Acridine ester NSP-DMAE-NHS, CAS 194357-64-7, is a luminescent reagent composed of a complex of acridine ester and a derivative of dimethylaminophenol (DMAE), which can react with catalase to produce a strong fluorescent signal. Due to the fluorescent signal emitted, many people currently use it as a fluorescent probe to facilitate the detection of catalase activity in biomolecules. So below, we will focus on analyzing its luminescence from four aspects.

1、 Chemical structure of acridine ester NSP-DMAE-NHS

Firstly, it is necessary to analyze the structure of the acridine ester NSP-DMAE-NHS. According to research, it contains a pyrrole ring and a dimethylaminophenol unit in the probe. The pyrrole ring has a nitrogen atom, while the dimethylaminophenol unit has a benzene ring and a methoxy group. The interaction between these functional groups can form a stable molecular structure and play an important role in the reaction.

2、 Fluorescence generation efficiency

Fluorescence generation efficiency is one of the important indicators, which differs significantly from other luminescent substances in the field of luminescence. Acrylamide ester emits light in 0.4 seconds after adding a promoter, and the intensity can reach its peak. It then emits light within 2 seconds, which can achieve rapid luminescence and improve efficiency for detection personnel.

3、 Fluorescence intensity

Fluorescence intensity is one of the important indicators for measuring sensitivity and specificity. For acridine ester, its maximum excitation fluorescence intensity is 2.8 × 10 ^ 4 times the reference wavelength (at this excitation wavelength of 525 nm). This intensity value is relatively high, which means that the presence of target molecules can be detected at lower concentrations. In addition, it should have high specificity because it can react with catalase without cross reacting with other molecules.

4、 Practical application areas

Due to the luminescent advantage of acridine ester, its application fields and effects are also very obvious. According to existing experimental research, it can be applied in chemiluminescence and immunoassay, as well as in nucleic acid and peptide detection. For example, it can be used to detect catalase activity in tumor cells through reagent kits and evaluate the functional status of immune cells. The accuracy and sensitivity of acridine ester NSP-DMAE-NHS have been fully demonstrated in these aspects.

As a professional supplier of luminescent reagents, Desheng produces six types of products in the acridine ester series and three types of products in the luminol series, which can effectively meet the different usage needs of customers. At the same time, due to the company's quality management requirements, all products are of analytical grade, with strong luminescence sensitivity and stability. If you have any relevant intentions, please feel free to contact us for supply at any time!