Gentier 48E

Gentier 48S

Gentier 48R

                      Gentier 96E

Gentier 96S

Gentier 96R

Gentier 96C

Thermal Block

Sample capacity(wells)

                                  48

96

Reaction Volume

5-100 pL

0-100 pL

Consumables

0.2ml 8-strip tubes ,0.2ml PCR single tube(Optical flat cap,clear tube)

0.2ml 96-well Plates

(unskirt) ; 0.2ml 8-strip tubes,0.2ml PCR single tube(Optical flat cap,clear,frosted,white tube)

Temperature range

0°C-100°C

0°C-100°C

Heating/cooling method

Peltier

Peltier

Max heating rate

8.0°C/sec

6.1°C/sec

Average heating rate

6.0°C/sec

4.5°C/sec

Max cooling rate

5.5°C/sec

5.0°C/sec

Average cooling rate

4.0°C/sec

2.8°C/sec

Temperature accuracy

±0.1 °C

±0.1 °C

Temperature uniformity

±0.1 °C

±0.1 °C

Gradient Range

1°C-40°C

N/A

1°C-40°C

1°C-40°C

N/A

1°C-40°C

N/A

Gradient block

8 row

8 row

12 row

12 row

Special temperature protocol

Gradient PCRXong PCR,Touch Down PCR

Long PCR.Touch Down PCR

Gradient PCRzLong PCR,Touch Down PCR

Gradient PCR,Long PCRlbuch Down PCR

Long PCR,Touch Down PCR

Gradient PCR,Long PCRlbuch Down PCR

Long PCR,Touch Down PCR

Heat Lid

Temperature range

Room Temperature-110°C

Room Temperature-110°C

Optical System

Excitation Source

4LEDs

2LEDs

6LEDs

4LEDs

Detector

Photodiode

Photodiode

Detection Position

Excitation and scan from lateral

Excitation and scan at top

Detection method

4 channels scanning at the same time, no edge effect.

6 channels scanning at the

same time, no edge effect.

Detection time

2 seconds for 48 wells for all channels

7 seconds for 96 wells for all channels

Range of excitation/ emission wavelengths(nm)

1  465 /510 (FAM,SYBR Green l,SYTO9,EvaGreen,LC Green)

2  527/ 563 (HEX,VIC,TET,JOE)

3  580 /616(ROX,Texas Red)

4  632/ 664( Cy5)

1  465 /510(FAM,SYBR Green I.SYTO9, EvaGreen,LC Green)

2  527/ 563 (HEX,VIC,TET,JOE)

1.465 /510(FAM,SYBR Green l,SYTO9,EvaGreen,LC Green)

2.527/ 563 (HEX,VIC,TET,JOE)                                                           3.580 /616 (ROX,Texas Red)

4.632/ 664( Cy5) 5.680 /730 (Alexa Fluor680) 6.465 /616 (FRET)

1.465 /510(FAM,SYBR Green l,SYTO9,EvaGreen,LC Green) 2.527/ 563 (HEX,VIC JET,JOE)                                                        3.580 /616 (ROX,Texas Red)

4.632/ 664( Cy5)

Probe

Taqman ProbezMolecular beacons probe, Scorpion probe

Taqman Probe,Molecular beacons probe,Scorpion probe ,FRET

Taqman Probe,Molecular beacons probe,Scorpion probe

Multiplexing

Up to 4 targets

Up to 2 targets

Up to 6 targets

Up to 4 targets

Fluorescence Linearity

r>0.990

rs0.990

Fluorescence Dynamic Range

Adjustable

Adjustable