RPR TEST KIT 100 TEST

SPECIMEN COLLECTION
Plasma unheated or heated serum may be used. Avoid hemolysis. Serum samples are reportedly stable for 5 days if stored at +4°C. plasma collected with EDTA is reportedly stable up to 24 hours.

REAGENT AND MATERIAL SUPPLIED
R1 : Carbon Antigen Suspension
R2 : Positive Control Serum
R3 : Negative Control serum

ACCESSORIES
20G Dispensing Needle (16µl/ drop)
Disposable Test cards
Disposable stirring rods
Disposable sample droppers

PROCEDURE : QUALITATIVE TEST
1. Bring all reagents and samples to room temperature.
2. Using the disposable sample dropper, dispense one drop of serum or plasma onto a separate circle on the test card. Use a fresh disposable sample dropper for each sample. Repeat step 2 using the positive and negative control sera.
3. Using the disposable stirring rod, spread the sample over the entire area of the test circle.
4. Mix the carbon antigen well and place one drop of "free fall" Antigen suspension onto each test specimen using 20G Dispensing Needle.
5. Place the card on a rotator and rotate for 8 minutes at 100 rpm. Immediately after 8 minutes rotation, read the results macroscopically in good light.

QUALITATIVE TEST RESULTS

REACTIVE: The presence of large aggregates in the center or the periphery of the test circle.

WEAKLY REACTIVE : The presence of small or fine aggregates.

NON-REACTIVE : Smooth grey appearance with no aggregates visible.

PROCEDURE:

QUANTITATIVE TEST

FOR EACH SPECIMEN TO BE TESTED :
1. Place 0.05 ml (50 µl) of 0.9% saline with a pipette into test circles, numbered 2 to 5. DO NOT SPREAD SALINE.
2. Place 0.05 ml (50 µl) of specimen onto test circle 1.
3. Place 0.05 ml (50 µl) of specimen onto the test circle 2. Prepare serial two-folds dilutions by drawing the mixture up and down the pipette 5-6 times (avoid any bubble formation). Transfer 0.05 ml from circle 2 to 3, to 4, to 5. Dispose 0.05 ml from circle 5 after mixing.
4. Using a new stirring rod for each specimen, start at highest dilution of serum (circle 5) and spread over entire area of test circle. Proceed to circles 4,3,2 and 1.
5. Follow steps 4 to 5 in the Procedure of qualitative test.

If the last dilution (circle 5) is reactive, the dilution series should be extended as follows.

1. Prepare a 1:50 dilution of non-reactive serum with on 0.9% saline. This is to be used for making 1:32 and higher dilutions of specimens to be quantitated. Dispense 50 µl onto circles numbered 7 to 10.
2. Prepare a 1:16 dilution of test specimen by adding 100 µl serum to 1500 µl of 0.9% saline. Mix thoroughly. Dispense 0.05 µl of 1:16 dilution of test specimens onto circles 6 and 7.
3. On circle 7, mix the sample and diluent using the disposable dropper by drawing the mixture up and down 5 to 6 times (avoid any bubble formation). Transfer 50 µl of the mixed sample to the next circle. Repeat the mixing procedure. Continue this serial dilution to circle.
4. Dispose 50 µl from circle 10 after mixing.

REFRENCES
Hunter, E.F., W.E. Deacon and P.E. Meyer, 1964. an approved FTA Test for Syphilis, the absorbtion Procedure (FTA-ABS).